British anchor runner Nethaneel Michell-Blake also dropped onto the ground, in blissful disbelief as his team took the important victory in a world lead time of 37.47 seconds, beating the American squad, consisting of new 100m victor Justin Gatlin and runner-up Christian Coleman, to second place in 37.52.
Since confirming his sprinting dominance with triple gold at the 2008 Beijing Olympics, Bolt spent nine seasons amassing 19 global golds, 13 of which have come in individual events.
Usain Bolt ended his stellar career in excruciating pain.
Gatlin defeated Bolt in the 100-meter relay last weekend, smoking the competition after finishing the race at 9.92 seconds to grab the gold medal at the World Athletic Championships. Rico Freimuth took silver with 8,564 points, while German teammate Kai Kazmirek was third with 8,488. We were hanging around inside the stadium for too long with our clothes off and i think that's where Usain Bolt's injury came from.
Five years after winning the 100-meter hurdles at the London Games, Pearson won the world title in 12.59 seconds.
France and China also qualified.
However, Blake - who missed out on a medal in the 100m, failed to make the 200m final and was an injury doubt in the hours leading up to the race - was overtaken around the bend by Jaylen Bacon and Daniel Talbot.
He hobbled and went down in agony as Britain kept on going to hold off the United States of America and take gold.
"To run it with Danny Talbot after such disappointment in London 2012, it is so special to come back", said Gemili, who was not selected for the individual races and missed an Olympic 200m bronze by three thousandths of a second past year. If he wins he will be the first athlete to have swept the event at youth, junior and senior level. Germany won the second heat in 42.34.
Bolt has amassed eight Olympic golds and 14 world medals, 11 of which are gold, the Jamaican having won two silvers in 2007 before his 100m bronze in London.
Mitchell Blake took the baton three metres up from America with Jamaica further adrift and the pressure was on to hold off the challenges of rising star Coleman and the fearsome Bolt.
Even Usain Bolt's perceived rival Justin Gatlin conceded the drop in temperature had an affect.