This is the first time the two met at Indian Wells, a match was in the making for 17 years.
Venus thus wasn't dwelling on her win Monday. On her big return, the sisters put on quite show of power at Indian Wells and fans could not get enough. She kept roaring back.
"I definitely know her well, but she definitely played a little bit better than she normally does", Serena said. "I'm from right down the street in Compton, California". "It's just the nerves, the anticipation you feel naturally".
The sisters arrived outside the stadium together on a golf cart then walked through the tunnel with Serena entering the court first with many in the crowd standing and cheering.
The two sisters resumed their rivalry, which began in second round of Australia Open 1998, on Tuesday in the third-round with Serena holding a 17-11 head-to-head record and having won eight of the last nine matches. Such was the case when they last squared off at the 2017 Australian Open, with Serena prevailing in the early stages of pregnancy.
The first two games alone took 14 minutes. Venus is seeded eighth.
She took the first game behind a swift 118 miles per hour serve, then held serve before breaking Serena in the sixth game to take a 4-2 lead.
But Vesnina never earned a set point as Kerber overcome a marathon first set - saving break points and reeling off the final three games.
"Being older again than Serena, myself, it's wonderful to see and I'm very happy for them, especially now watching Serena's comeback since the baby".
Serena and Venus Williams won't be meeting in a grand slam Monday and their match won't be a final, either.
Some great defence from Serena drew an overhead error from Venus and suddenly the younger sibling had a break point. Venus' forehand was long and she double-faulted away the game to make it 5-3.
Serena held for 4-5, forcing Venus to try and serve out the match for a second time.
She joined American upset winners Sachia Vickery, who beat No 3 seed Garbine Muguruza; 16-year-old wild card Amanda Anisimova, who beat No 23 seed Anastasia Pavlyuchenkova; and wild-card teenager Caroline Dolehide, a three-set victor over No 30 Dominika Cibulkova on Friday.